期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2019.110428
关键词
Nanoerythrosome; Ghost; Protein network; Protein scaffolding; Protein - lipid interactions
资金
- National Research Development and Innovation Office (Hungary) [PD 121326, NVKP_16-1-2016-0007]
- HunProtExc
- Protein research program of Hungarian Academy of Sciences (MEDinPROT)
- Janos Bolyai Research Fellowship of the Hungarian Academy of Sciences
A peculiar polygonal protein scaffolding that resembles to spectrin-based skeleton of red blood cells can be reconstructed on the outer surface of vesicle-like nanoerythrosomes. The approximately 130 nm sized nanoerythrosomes are produced from red blood cell ghosts by addition of phospholipids (dipalmitoylphosphatidylcholine, DPPC). The scaffolding, constructed from the structural proteins of the cell membrane skeleton, covers the whole object resulting an enhanced stiffness. The protein pattern of the scaffolding is thermosensitive, reversible transformable in the biologically relevant temperature range. When the lipid additive is changed from DPPC to lysophospholipid (LPC), the protein network/scaffolding ceases to exist. By the variation of lipid type and ratio, a tailoring of the nanoerythrosomes can be achieved. During the tailoring process nanoerythrosomes or micelles, in a wide size range from 200 to 30 nm, are produced.
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