Ultrafast Dynamics at the Lipid-Water Interface: DMSO Modulates H-Bond Lifetimes

Dimethyl sulfoxide (DMSO) is a common cosolvent and cryopreservation agent used to freeze cells and tissues. DMSO alters the H-bond structure of water, but its interactions with biomolecules and, specifically, with biological interfaces remain poorly understood. Here we investigate the effects of DMSO on the H-bond dynamics at the lipid- water interface using a combination of ultrafast two-dimensional infrared (2D IR) spectroscopy and molecular dynamics simulations. Ester carbonyl absorption spectra show that DMSO dehydrates the interface, and simulations show that the area per lipid is decreased. Ultrafast 2D IR spectra measure the time scales of frequency fluctuations at the ester carbonyl positions located precisely between the hydrophobic and hydrophilic regions of the membrane. 2D IR measurements show that low DMSO concentrations (<10 mol %) induce similar to 40% faster H-bond dynamics compared with pure water, whereas increased concentrations (>10-20 mol %) once again slow down the dynamics. This slow-fast-slow trend is described in terms of two different solvation regimes. Below 10 mol %, DMSO weakens the interfacial H bond, leading to faster bulk-like dynamics, whereas above 10 mol %, water molecules become relatively immobilized as the H-bond networks becoming disrupted by the H-bond donor/acceptor imbalance at the interface. These studies are an important step toward characterizing the environments around lipid membranes, which are essential to numerous biological processes.