期刊
CHEMICAL ENGINEERING JOURNAL
卷 291, 期 -, 页码 287-297出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2016.01.104
关键词
Lipase; Hybrid nanoflower; Coordination; Immobilized enzyme; Precipitation reaction
资金
- State Key Program of National Natural Science of China [51433008]
- Natural Science Foundation of Shaanxi Province [2015JQ2055]
- Basic Research Fund of Northwestern Polytechnical University [3102014JCQ01094, 3102014ZD]
A facile and rapid method is reported in this paper to prepare a novel immobilized enzyme named lipase/Zn-3(PO4)(2) hybrid nanoflower. The growth mechanism of the nanoflower has been studied in detail and can be described as the following four steps: crystallization and coordination, in-situ precipitation, self-assembly, size growth. Addition amount of lipase, reaction temperature and stirring form affect the morphology and lipase content of the hybrid nanoflower. Besides, the catalytic performance of lipase/Zn-3(PO4)(2) hybrid nanoflower was investigated and the optimal catalytic conditions have been found. The maximum enzyme activity was 855 +/- 13 U/g. In comparison with the free lipase, the enzyme activity increment of hybrid nanoflower is 147%. Meanwhile, the unique nanostructure makes lipase/Zn-3(PO4)(2) hybrid nanoflower an excellent operational stabilizer. The results indicate that the well designed materials should be useful in industrial enzyme catalysis. (C) 2016 Elsevier B.V. All rights reserved.
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