期刊
JOURNAL OF TRANSLATIONAL MEDICINE
卷 18, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12967-020-02360-6
关键词
Metagenomic next-generation sequencing; Central nervous system infection; Application; Etiology; Diagnose
资金
- New and Advanced Technology Project of Shanghai Municipal Hospital [SHDC12017104]
- Key Technologies Research and Development Program for Infectious Diseases of China [2018ZX10305-409-001-003]
Background Accurate etiology diagnosis is crucial for central nervous system infections (CNS infections). The diagnostic value of metagenomic next-generation sequencing (mNGS), an emerging powerful platform, remains to be studied in CNS infections. Methods We conducted a single-center prospective cohort study to compare mNGS with conventional methods including culture, smear and etc. 248 suspected CNS infectious patients were enrolled and clinical data were recorded. Results mNGS reported a 90.00% (9/10) sensitivity in culture-positive patients without empirical treatment and 66.67% (6/9) in empirically-treated patients. Detected an extra of 48 bacteria and fungi in culture-negative patients, mNGS provided a higher detection rate compared to culture in patients with (34.45% vs. 7.56%, McNemar test, p < 0.0083) or without empirical therapy (50.00% vs. 25.00%, McNemar test, p > 0.0083). Compared to conventional methods, positive percent agreement and negative percent agreement was 75.00% and 69.11% separately. mNGS detection rate was significantly higher in patients with cerebrospinal fluid (CSF) WBC > 300 * 10(6)/L, CSF protein > 500 mg/L or glucose ratio <= 0.3. mNGS sequencing read is correlated with CSF WBC, glucose ratio levels and clinical disease progression. Conclusion mNGS showed a satisfying diagnostic performance in CNS infections and had an overall superior detection rate to culture. mNGS may held diagnostic advantages especially in empirically treated patients. CSF laboratory results were statistically relevant to mNGS detection rate, and mNGS could dynamically monitor disease progression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据