Correspondence analysis of bio-refractory compounds degradation and microbiological community distribution in anaerobic filter for coking wastewater treatment

Coking wastewater usually contains lots of mutative bio-refractory compounds, which causes the difficulties for the biological coking wastewater treatment. In this work, we employed Gas Chromatography-Mass Spectrometer to determine organic compounds concentration along different heights of anaerobic filter and 16SrRNA high-throughput gene sequencing analysis to reveal microbial community distribution. As results, 360 genera were detected in the reactor, where Acfpbacter, Proteiniphilum, Pseudomonas, Comamonas, Sedimentibacter, Enterobacter and Dysgonomonas dominated the microbiological community. They were found to have obvious correlations with the degradation of some bio-refractory compounds including phenol, methylphenol, dimethylphenol, naphthol, triphenylene and indole in coking wastewater. Specially, phenol degradation has a high coefficient with Enterobacter, Pseudomonas and Sedimentibacter. Methylphenol degradation exhibits the good relativity with Enterobacter and Sedimentibacter. Besides the well-known Enterobacter, Dysgonomonas, Pseudomonas and Comamonas for phenol and methylphenol degradation, most importantly, we found the high correlation coefficients of Sedimentibacter to phenol, methylphenol, dimethylphenol and naphthol degradation. The new finding would be benefit for the identification of Sedimentibacter for phenol, methylphenol, dimethylphenol and naphthol degradation, and the isolation of related bacteria for bioaugmentation. This study is useful to reveal black box model of coking wastewater biological treatment and provides another way to investigate functional bacteria to degrade bio-refractory compounds. (C) 2016 Published by Elsevier B.V.