期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 142, 期 16, 页码 7542-7554出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.0c01191
关键词
-
资金
- Japan Science and Technology Agency under Precursory Research for Embryonic Science and Technology (PRESTO) [JPMJPR14F1, JPMJPR18G1]
- MEXT Japan
- JSPS KAKENHI [JP18K19297, JP26220602, JP26286028, JP18H01838, JP15H05931, JP18H05474, JP18J10796, JP19H05380, JP19K16089]
- Japan Science and Technology Agency under Core Research for Evolutional Science and Technology (JST CREST, Japan) [JPMJCR1333]
- Japan Society for Promotion of Science under the Funding Program for Next-Generation World Researchers (NEXT Program) [LS072]
- PRIME from Japan Agency for Medical Research and Development [19gm6110022h001]
- MEXT Quantum Leap Flagship Program (MEXT Q-LEAP) [JPMXS0118067395]
- Sasakawa Scientific Research Grant
The rotation of an object cannot be fully tracked without understanding a set of three angles, namely, roll, pitch, and yaw. Tracking these angles as a three-degrees-of-freedom (3-DoF) rotation is a fundamental measurement, facilitating, for example, attitude control of a ship, image stabilization to reduce camera shake, and self-driving cars. Until now, however, there has been no method to track 3-DoF rotation to measure nanometer-scale dynamics in biomolecules and live cells. Here we show that 3-DoF rotation of biomolecules can be visualized via nitrogen-vacancy centers in a fluorescent nanodiamond using a tomographic vector magnetometry technique. We demonstrate application of the method to three different types of biological systems. First, we tracked the rotation of a single molecule of the motor protein F1-ATPase by attaching a nanodiamond to the.-subunit. We visualized the 3-step rotation of the motor in 3D space and, moreover, a delay of ATP binding or ADP release step in the catalytic reaction. Second, we attached a nanodiamond to a membrane protein in live cells to report on cellular membrane dynamics, showing that 3D rotational motion of the membrane protein correlates with intracellular cytoskeletal density. Last, we used the method to track nonrandom motions in the intestine of Caenorhabditis elegans. Collectively, our findings show that the method can record nanoscale 3-DoF rotation in vitro, in cells, and even in vivo. 3-DoF rotation tracking introduces a new perspective on microscopic biological samples, revealing in greater detail the functional mechanisms due to nanoscale dynamics in molecules and cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据