期刊
JOURNAL OF SEPARATION SCIENCE
卷 43, 期 13, 页码 2521-2528出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201901164
关键词
isoquinoline alkaloids; preparative separation; positive charge; traditional Tibetan medicine
资金
- Applied Basic Research Project of Qinghai Province [2016-ZJ-714]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2017471]
- International Cooperation Project of Qinghai Province [2019-HZ-805]
- Major New Drug Creation Special Projects [2019ZX09201001]
- Innovation Platform for the Development and Construction of Special Project of Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province [2017-ZJ-Y19]
Positively charged reversed-phase liquid chromatography was employed for the efficient preparative separation of isoquinoline alkaloids from Corydalis impatiens. Ten commercially available columns were compared for isoquinoline alkaloids analysis. While tailing, overloading, lower resolution, and buffer salts limited the application in purification of isoquinoline compounds of many of these columns, one positively charged reversed-phase C18 column (XCharge C18) overcame these drawbacks, allowing for favorable separation resolution, even when loading isoquinoline compounds on a larger, preparative scale. The general separation process is as follows. First, isoquinoline alkaloids are enriched with Corydalis impatiens extract via a middle chromatogram isolated gel column. After column selection, separation is performed on an XCharge C18 analytical column, from which two evident chromatographic peaks are readily obtained. Finally, two isoquinoline alkaloids (protopine and corydamine) are selectively purified on the XCharge C18 preparative column. These results demonstrate that a middle chromatogram isolated gel column coupled with positively charged reversed-phase liquid chromatography is effective for the preparative separation of isoquinoline alkaloids from Corydalis impatiens.
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