期刊
JOURNAL OF PROTEOME RESEARCH
卷 19, 期 7, 页码 2553-2562出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.0c00022
关键词
peptidisc; membrane proteomics; membrane proteome; Sec translocon; SecDFyajC depletion; SecA; YibN
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) [DG- 04241]
- BC Knowledge Development Fund
- Genome BC (PanCanadian Proteomics Centre) [214PRO]
Characterization of the integral membrane proteome by mass spectrometry (MS) remains challenging due its high complexity and inherent insolubility. In a typical experiment, the cellular membranes are isolated, the proteins are solubilized and fractionated, and the detergent micelles are removed before MS analysis. Detergents are not compatible with mass spectrometry, however, and their removal from biological samples often results in reduced protein identification. As an alternative to detergents, we recently developed the peptidisc membrane mimetic, which allows entrapment of the cell envelope proteome into water-soluble particles, termed a peptidisc library. Here, we employ a His-tagged version of the peptidisc peptide scaffold to enrich the reconstituted membrane proteome by affinity chromatography. This purification step reduces the sample complexity by depleting ribosomal and soluble proteins that often cosediment with cellular membranes. As a result, the peptidisc library is enriched in low-abundance membrane proteins. We apply this method to survey changes in the membrane proteome upon depletion of the SecDFyajC complex, the ancillary subunit of the Sec translocon. In the depleted strain, we detect increased membrane localization of the motor ATPase SecA, along with increased levels of an unannotated inner membrane protein, YibN. Together, these results demonstrate the utility of the peptidisc for global purification of membrane proteins and for monitoring change in the membrane proteome.
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