期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 184, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2020.113173
关键词
Solvent extraction; Microwave-assisted extraction; Rosmarinic acid; Lamiaceae family; HPLC
资金
- New National Excellence Program of the Ministry for Innovation and Technology [UNKP-19-3-I-SZE-11]
- European Union [EFOP-3.6.2-16-2017-00012]
- European Social Fund [EFOP-3.6.2-16-2017-00012]
The goal of this study was to improve the extraction efficiency of rosmarinic acid (RA) from Lamiaceae herbs (lemon balm, peppermint, oregano, rosemary, sage, and thyme) using various extraction techniques (maceration with stirring, MACs; heat reflux, HRE; and microwave-assisted extraction, MAE) and extraction conditions (solvent acidity, solvent type, extraction time and temperature). The RA content was measured by high-performance liquid chromatography with diode-array detection (HPLC-DAD) under test conditions. Our results showed that extraction with acidified aqueous ethanol (EtOH-H2O-HCl, 70:29:1, v/v/v) was the best choice for the recovery of RA compared to other solvent systems. Further study suggested the following optimal extraction times for the different techniques: 120 min at 25 degrees C with MAC(s), 15 min at boiling point with HRE, and 5 min at 50 degrees C and 80 degrees C with MAE. Based on our results, we demonstrated that by careful adjustment of the extraction conditions, it is possible to set up a single extraction protocol to extract RA from different plants. (C) 2020 The Author. Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据