Optical analysis of bone tissue by Raman spectroscopy in experimental osteoporosis and its correction using allogeneic hydroxyapatite

The results of experimental studies of bone tissue in models of osteoporosis are presented, and the effectiveness of the correction of this pathological condition using allogeneic hydroxyapatite is evaluated by Raman spectroscopy. During the studies, Raman spectra of the cancellous and cortical bone tissues were obtained and the spectra were deconvolved into bands using the Gaussian function. The introduced coefficients make it possible to evaluate the effectiveness of various doses of hydroxyapatite (10, 20, 30 mg/kg) for the correction of osteoresorption in models of osteoporosis by ovariectomy. It was established that, in contrast to the cortical bone, pronounced changes in the coefficients I-1069 /I-1739, I-850/I-1739, and I-1243/I-1739 were not observed in the cancellous bone. The introduction of a suspension of hydroxyapatite showed that during the indicated treatment period, the consequences of ovariectomy can be reversed by 40%-60% for the cancellous bone and almost completely for the cortical bone (70%-100%). (C) 2020 Optical Society of America