4.2 Article

Histological response and expression of collagen, metalloproteinases MMP-1 and MMP-9 and tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2 in fetal membranes following open intrauterine surgery: an experimental study

期刊

JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
卷 35, 期 7, 页码 1301-1309

出版社

TAYLOR & FRANCIS LTD
DOI: 10.1080/14767058.2020.1752654

关键词

Collagen; fetal membrane repair; fetal membrane healing; myelomeningocele; open fetal surgery; matrix metalloproteinases

资金

  1. CoordenacAo de Aperfeicoamento de Pessoal de Nivel Superior - CAPES, Brazil
  2. NSC

向作者/读者索取更多资源

This study evaluated the repair process of fetal membranes after open fetal surgery for myelomeningocele. The results showed increased collagen, altered expression of MMP-9 and TIMP-1, and the repair process was also influenced by the number of previous pregnancies and gestational age at the time of surgery.
Objective: To characterize aspects of the repair process by evaluating the tissue collagen density, metalloproteinases and tissue inhibitor of matrix metalloproteinases in the fetal membranes following open fetal surgery for myelomeningocele (MMC). Design: Experimental. Setting: Two Brazilian hospitals in 2013-2014. Population: 30 fetal membranes collected after elective cesarean deliveries, in patients who underwent open fetal surgery for MMC intrauterine repair. Methods: Regions within and surrounding the scar area and regions distant from the surgical site were evaluated for collagen concentration and expression of MMP-1, MMP-9, TIMP-1 and TIMP-2. Results: Collagen was increased in regions of scar formation (14.4 +/- 2.7%) as compared to unaffected regions (8.0 +/- 1.9%) (p < .001). The mean score of MMP-9 in the area of both the scar and suture was also increased above that observed in normal regions (p < .05). Conversely, MMP-1 was reduced in the scar when compared to the normal region and the area adjacent to the scar (suture region) (p < .05). TIMP-1 was increased in the suture region compared to the normal region (p < .05) while TIMP-2 was reduced in the scar region when compared to the other two regions (p < .05). The membrane repair process was also influenced by the number of previous pregnancies and gestational age at the time of surgery. Conclusion: Reparative activity of the fetal membrane after open fetal surgery involves up-regulation of collagen production and differential involvement of MMPs and TIMPs.

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