期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 109, 期 2, 页码 309-325出版社
OXFORD UNIV PRESS
DOI: 10.1002/JLB.4HI0420-285RR
关键词
CD40 signaling; CD40; Ebola virus; filovirus; IL-12; innate immunity; IFN-gamma; M phi; peritoneum; RNA virus; TRAF6; virus restriction
资金
- NIH [R21 AI139902, R21 AI144215, T32GM007337, AI119163, R01AI127481, R01AI125446, AI114776, RO1AI124093, P30 CA086862]
- University of Iowa Department of Microbiology and Immunology Developmental Grant Award
- VA Merit Review [I01 BX001702]
- Carver College of Medicine
- Holden Comprehensive Cancer Center
- Iowa City Veteran's Administration Medical Center
CD40 expression in peritoneal M phi s restricts early infection of a broad range of RNA viruses. CD154/CD40 interactions stimulate IL-12 production, leading to IFN-gamma production and proinflammatory polarization of M phi s, protecting the cells from infection. These CD40-dependent events protect mice against virus challenge.
Many acute viral infections target tissue M phi s, yet the mechanisms of M phi-mediated control of viruses are poorly understood. Here, we report that CD40 expressed by peritoneal M phi s restricts early infection of a broad range of RNA viruses. Loss of CD40 expression enhanced virus replication as early as 12-24 h of infection and, conversely, stimulation of CD40 signaling with an agonistic Ab blocked infection. With peritoneal cell populations infected with the filovirus, wild-type (WT) Ebola virus (EBOV), or a BSL2 model virus, recombinant vesicular stomatitis virus encoding Ebola virus glycoprotein (rVSV/EBOV GP), we examined the mechanism conferring protection. Here, we demonstrate that restricted virus replication in M phi s required CD154/CD40 interactions that stimulated IL-12 production through TRAF6-dependent signaling. In turn, IL-12 production resulted in IFN-gamma production, which induced proinflammatory polarization of M phi s, protecting the cells from infection. These CD40-dependent events protected mice against virus challenge. CD40(-/-) mice were exquisitely sensitive to intraperitoneal challenge with a dose of rVSV/EBOV GP that was sublethal to CD40(+/+) mice, exhibiting viremia within 12 h of infection and rapidly succumbing to infection. This study identifies a previously unappreciated role for M phi-intrinsic CD40 signaling in controlling acute virus infection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据