An autocatalytic DNA machine with autonomous target recycling and cascade circular exponential amplification for one-pot, isothermal and ultrasensitive nucleic acid detection

An autonomous target recycling and cascade circular exponential amplification strategy was proposed to construct an autocatalytic DNA machine, which afforded one-pot, isothermal and ultrasensitive detection of nucleic acids. A low detection limit of 0.61 fM toward the target DNA with an excellent selectivity could be obtained.