4.5 Article

Characteristics of pressurized hot water extract from abalone muscle and the antioxidant ability during simulated digestion in vitro

期刊

JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
卷 57, 期 11, 页码 4076-4083

出版社

SPRINGER INDIA
DOI: 10.1007/s13197-020-04441-2

关键词

Abalone muscle; Pressurized hot water extract; Protein; Polysaccharide; In vitro digestion; Antioxidant activity

资金

  1. National Natural Science Fund [31571835]
  2. Fujian Key Project of Natural Science Fund [2019J02013]
  3. Special Scientific Research Fund of Marine Public Welfare [201405016]
  4. Xiamen Science and Technology Project [3502Z20173032]

向作者/读者索取更多资源

The physicochemical properties of pressurized hot-water extract (PHE) from abalone muscle and the antioxidant activities of its digestive products were investigated in this study. The PHE contained protein of 49.58% and carbohydrate of 41.95%. After ethanol graded precipitation, most of carbohydrate and protein in PHE were successively remained in 40% ethanol precipitate (EP40) and 80% ethanol precipitate (EP80), respectively. High proportions of Glu and Gly were found in the PHE, EP40 and EP80, but the proportion of Ala in ethanol soluble extract (ESE) reached up to 46.00%. Both PHE and EP40 were rich in glucose, while galactose and glucose were main monosaccharides in the EP80 and ESE. Based on the results of SDS-PAGE and HPLC, high molecular weight components from PHE were precipitated in the EP40, but oligopeptides and free amino acids were fractionated in EP80 and ESE. Among the PHE and the digestive products, the highest antioxidant ability was found to be EP80 hydrolysate, the IC(50)values of which for scavenging activity on hydroxyl radical, DMPD radical and ABTS radical were 1.05 mg/mL, 1.40 mg/mL and 0.56 mg/mL, respectively. It is concluded that carbohydrate of abalone muscle was dissolved more easily into hot water than protein, and protein hydrolysate of PHE might play an important role in antioxidant activity of gastrointestinal digestion.

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