Antibacterial, Cytotoxicity and Larvicidal Activity of Green Synthesized Selenium Nanoparticles Using Penicillium corylophilum

Anopheles mosquitoes became one of the biggest problems threaten human life through causing malaria disease. Hereby, Selenium nanoparticles (Se-NPs) as anti-victor malaria were fabricated for the first time through Penicillium corylophilum in presence of ascorbic acid as a reducing agent. Antibacterial versus pathogenic Gram +Ve and Gram -Ve bacteria, as well, in vitro cytotoxicity against two types of cell lines (normal Wi 38 and cancer Caco-2) were explored prolonging with their larvicidal activity towards Anopheles stephensi mosquitoes. Se-NPs is characterized by UV-Vis spectroscopy, FT-IR, TEM, EDX, XRD, DLS. Results affirmed the ability of Penicillium corylophilum to build up Se-NPs in spherical shape with average size 29.1-48.9 nm. In addition, Se-NPs exhibited broad spectrum activity against pathogenic Gram +Ve and Gram -Ve bacteria, while the results obtained from cytotoxicity evaluation signified clearly that, alteration occurred in the cells was represented as loss of their typical shape, partial or complete loss of monolayer, granulation, shrinking or cell rounding with IC50 value of 171.8 and 104.3 ppm Wi 38 and Caco-2 cell lines respectively, which revealing the high toxicity of Se-NPs towards cancer cells compared with normal cells. However, Se-NPs displayed larvicidal activity against causative malaria vector Anopheles stephensi.

Automated summary

Anopheles mosquitoes pose a serious threat to human life by transmitting malaria, and Selenium nanoparticles (Se-NPs) synthesized by Penicillium corylophilum have shown antibacterial properties against both Gram +Ve and Gram -Ve bacteria, as well as high toxicity towards cancer cells. Additionally, Se-NPs exhibit larvicidal activity against Anopheles mosquitoes.