期刊
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
卷 95, 期 12, 页码 3193-3201出版社
WILEY
DOI: 10.1002/jctb.6497
关键词
l-lactic acid; food waste; pilot-scale fermentation; lactic acid bacteria; Lactobacillus paracaseisubsp; paracasei2
类别
资金
- National Key RAMP
- D Program of China [2019YFC1900902]
- International Science and Technology Innovation Cooperation Key Project [2016YFE0127800]
- National Natural Science Foundation of China [51708024, 51978047]
- Beijing Natural Science Foundation [8182035]
- Fundamental Research Funds for the Central Universities [FRF-TP-19-021A2]
BACKGROUND Lactic acid (LA) is a valuable industrial chemical with wide applications. Presently, the demand for optically purel-LA has grown considerably as a result of its applications in the food and pharmaceutical industries, and biodegradable polylactic acid production. The production ofl-LA from food waste (FW) has been discussed in some studies; however, most fermentation is performed under sterile conditions and with the addition of specific lactic acid bacteria. Completely autoclaving organic solid wastes is difficult and economically unfeasible. The aim of this work was to investigate pilot-scale LA production from FW under sterile and nonsterile conditions. RESULTS When FW was fermented without sterilization and inoculation, the maximum yield of LA was 0.43 g LA g(-1)total solid (TS) and the proportion ofl-LA was only approximate to 50%. A newly isolated strain,Lactobacillus paracaseisubsp.paracasei2, which was isolated and identified from FW, was used to improve the proportion ofl-LA in total LA. When nonsterile FW was inoculated with this strain, the proportion ofl-LA increased to 72.3%. After FW was sterilized and inoculated with this strain, the proportion ofl-LA was 98.6% and 98.8% under intermittent and real-time regulation of pH level, respectively. The maximum LA yield could increase to 0.55 g LA g(-1)TS. CONCLUSION Pilot-scale LA production from FW was determined by comparing the isomer purity ofl-LA between sterilization and nonsterilization and between inoculation and noninoculation, which provided an important strain and fermentation type for production of high concentration ofl-LA. (c) 2020 Society of Chemical Industry
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