期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 24, 期 13, 页码 7175-7186出版社
WILEY
DOI: 10.1111/jcmm.15181
关键词
adipogenesis; BADLNCR1; bovine; GLRX5; KLF2
资金
- National Natural Science Foundation of China [31672400, 31772574]
- Program of National Beef Cattle and Yak Industrial Technology System [CARS-37]
Adipogenesis is a complex cellular process, which needs a series of molecular events, including long non-coding RNA (lncRNA). In the present study, a novel lncRNA named BADLNCR1 was identified as a regulator during bovine adipocyte differentiation, which plays an inhibitory role in lipid droplet formation and adipogenic marker gene expression. CHIPR-seq data demonstrated a potential competitive binding motif between BADLNCR1 and sterol regulatory element-binding proteins 1 and 2 (SREBP1/2). Dual-luciferase reporter assay indicated target relationship between KLF2 and BADLNCR1. Moreover, after the induction of KLF2, the expression of adipogenic gene reduced, while the expression of BADLNCR1 increased. Real-time quantitative PCR (qPCR) showed that BADLNCR1 negatively regulated mRNA expression of GLRX5 gene, a stimulator of genes that promoted formation of lipid droplets and expression of adipogenic genes. GLRX5 could partially reverse the effect of BADLNCR1 in bovine adipocyte differentiation. Dual-luciferase reporter assay stated that BADLNCR1 significantly reduced the enhancement of C/EBP alpha on promoter activity of GLRX5 gene. Furthermore, CHIP-PCR and CHIRP-PCR confirmed the suppressing effect of BADLNCR1 on binding of C/EBP alpha to GLRX5 promoter. Collectively, this study revealed the molecular mechanisms underlying the negative regulation of BADLNCR1 in bovine adipogenic differentiation.
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