期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 295, 期 19, 页码 6710-6720出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA119.010438
关键词
guanine nucleotide exchange factor (GEF); gene knockout; microglia; astrocyte; migration; neurodegeneration; DOCK10; DOCK11; DOCK9
资金
- Japan Society for the Promotion of Science KAKENHI [JP20K07751, JP17K07123, JP20K09820, JP20K18404, JP19K09943, JP18K19625]
- Taiju Life Social Welfare Foundation
- Takeda Science Foundation
The DOCK-D (dedicator of cytokinesis D) family proteins are atypical guanine nucleotide exchange factors that regulate Rho GTPase activity. The family consists of Zizimin1 (DOCK9), Zizimin2 (DOCK11), and Zizimin3 (DOCK10). Functions of the DOCK-D family proteins are presently not well-explored, and the role of the DOCK-D family in neuroinflammation is unknown. In this study, we generated three mouse lines in which DOCK9 (DOCK9(?/?)), DOCK10 (DOCK10(?/?)), or DOCK11 (DOCK11(?/?)) had been deleted and examined the phenotypic effects of these gene deletions in MOG(35?55) peptide-induced experimental autoimmune encephalomyelitis, an animal model of the neuroinflammatory disorder multiple sclerosis. We found that all the gene knockout lines were healthy and viable. The only phenotype observed under normal conditions was a slightly smaller proportion of B cells in splenocytes in DOCK10(?/?) mice than in the other mouse lines. We also found that the migration ability of macrophages is impaired in DOCK10(?/?) and DOCK11(?/?) mice and that the severity of experimental autoimmune encephalomyelitis was ameliorated only in DOCK10(?/?) mice. No apparent phenotype was observed for DOCK9(?/?) mice. Further investigations indicated that lipopolysaccharide stimulation up-regulates DOCK10 expression in microglia and that microglial migration is decreased in DOCK10(?/?) mice. Up-regulation of C?C motif chemokine ligand 2 (CCL2) expression induced by activation of Toll-like receptor 4 or 9 signaling was reduced in DOCK10(?/?) astrocytes compared with WT astrocytes. Taken together, our findings suggest that DOCK10 plays a role in innate immunity and neuroinflammation and might represent a potential therapeutic target for managing multiple sclerosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据