期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 295, 期 19, 页码 6594-6604出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA120.013316
关键词
DNA transformation; protein structure; type IV pili; nuclear magnetic resonance (NMR); phylogenetics; DNA uptake; Streptococcus sanguinis; type IV filaments; type IV pilin
资金
- Medical Research Council [MR/P022197/1, 2449/2016]
- INCEPTION project [PIA/ANR-16-CONV-0005]
- MRC [MR/P022197/1, MR/P028225/1] Funding Source: UKRI
Type IV filaments (T4F), which are helical assemblies of type IV pilins, constitute a superfamily of filamentous nanomachines virtually ubiquitous in prokaryotes that mediate a wide variety of functions. The competence (Com) pilus is a widespread T4F, mediating DNA uptake (the first step in natural transformation) in bacteria with one membrane (monoderms), an important mechanism of horizontal gene transfer. Here, we report the results of genomic, phylogenetic, and structural analyses of ComGC, the major pilin subunit of Com pili. By performing a global comparative analysis, we show that Com pili genes are virtually ubiquitous in Bacilli, a major monoderm class of Firmicutes. This also revealed that ComGC displays extensive sequence conservation, defining a monophyletic group among type IV pilins. We further report ComGC solution structures from two naturally competent human pathogens, Streptococcus sanguinis (ComGC(SS)) and Streptococcus pneumoniae (ComGC(SP)), revealing that this pilin displays extensive structural conservation. Strikingly, ComGC(SS) and ComGC(SP) exhibit a novel type IV pilin fold that is purely helical. Results from homology modeling analyses suggest that the unusual structure of ComGC is compatible with helical filament assembly. Because ComGC displays such a widespread distribution, these results have implications for hundreds of monoderm species.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据