期刊
CHEMBIOCHEM
卷 17, 期 20, 页码 1892-1899出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201600303
关键词
antibodies; Fab fragments; monobodies; nanobodies; protein engineering
资金
- W. M. Keck Foundation
- Colorado Center for Drug Discovery
The immune system utilizes antibodies to recognize foreign or disease-relevant receptors, initiating an immune response to destroy unwelcomed guests. Because researchers can evolve antibodies to bind virtually any target, it is perhaps unsurprising that these reagents, and their small-molecule conjugates, are used extensively in clinical and basic research environments. However, virtues of antibodies are countered by significant challenges. Foremost among these is the need for expression in mammalian cells (largely due to often necessary post-translational modifications). In response to these challenges, researchers have developed an array of minimalist antibodies and mimetics, which are smaller, more stable, simpler to express in Escherichia coli, and amendable to laboratory evolution and protein engineering. Here we describe these scaffolds and discuss recent applications of minimalist antibodies and mimetics.
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