期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 295, 期 24, 页码 8155-8163出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA119.010911
关键词
membrane transport; cell metabolism; tricarboxylic acid cycle (TCA cycle) (Krebs cycle); computer modeling; bioenergetics; citrate; NaCT; slc13; SLC26
资金
- United States-Israel Binational Science Foundation Grant [2015003]
- Israel Science Foundation [271/16, 2164/16]
Metabolite transport across cellular membranes is required for bioenergetic processes and metabolic signaling. The solute carrier family 13 (slc13) transporters mediate transport of the metabolites succinate and citrate and hence are of paramount physiological importance. Nevertheless, the mechanisms of slc13 transport and regulation are poorly understood. Here, a dynamic structural slc13 model suggested that an interfacial helix, H4c, which is common to all slc13s, stabilizes the stationary scaffold domain by anchoring it to the membrane, thereby facilitating movement of the SLC13 catalytic domain. Moreover, we found that intracellular determinants interact with the H4c anchor domain to modulate transport. This dual function is achieved by basic residues that alternately face either the membrane phospholipids or the intracellular milieu. This mechanism was supported by several experimental findings obtained using biochemical methods, electrophysiological measurements inXenopusoocytes, and fluorescent microscopy of mammalian cells. First, a positively charged and highly conserved H4c residue, Arg(108), was indispensable and crucial for metabolite transport. Furthermore, neutralization of other H4c basic residues inhibited slc13 transport function, thus mimicking the inhibitory effect of the slc13 inhibitor, slc26a6. Our findings suggest that the positive charge distribution across H4c domain controls slc13 transporter function and is utilized by slc13-interacting proteins in the regulation of metabolite transport.
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