期刊
CHEMBIOCHEM
卷 17, 期 5, 页码 403-406出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500646
关键词
5-hydroxymethylcytosine; 5-methylcytosine; DNA recognition; high-throughput sequencing; sequence determination; Tet
Tet (ten-eleven translocation) family proteins have the ability to oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC). However, the oxidation reaction of Tet is not understood completely. Evaluation of genomic-level epigenetic changes by Tet protein requires unbiased identification of the highly selective oxidation sites. In this study, we used high-throughput sequencing to investigate the sequence specificity of mC oxidation by Tet1. A 6.6x10(4)-member mC-containing random DNA-sequence library was constructed. The library was subjected to Tet-reactive pulldown followed by high-throughput sequencing. Analysis of the obtained sequence data identified the Tet1-reactive sequences. We identified mCpG as a highly reactive sequence of Tet1 protein.
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