期刊
CHEMBIOCHEM
卷 17, 期 22, 页码 2118-2122出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201600490
关键词
ABC transporters; Abcg2; cell recognition; CDg13; fluorescent probes; neural stem/progenitor cells
资金
- A*STAR (Agency for Science, Technology and Research, Singapore) Biomedical Research Council
- Joint Council Office (JCO) Development Program, A*STAR [1334k00083]
Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2(low) cells from heterogeneous cell populations.
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