期刊
CHEMBIOCHEM
卷 17, 期 15, 页码 1403-1406出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201600258
关键词
glycocalyxs; glycopeptides; glycosylation; lectins; vesicles
资金
- Danish Research Council (Innovationsfonden)
- German Federal Ministry of Education and Research (BMBF) in the framework of the EU ERASyn-Bio project SynGlycTis
- Swedish Research Council (SSF)
- Excellence Initiative of the German Research Foundation [EXC 294]
- Ministry of Science Research and the Arts Baden-Wurttemberg [Az: 33-7532.20]
- European Research Council [ERC-2011-StG 282105]
Synthetic minimal membrane systems are extremely useful for better understanding of complex cellular structures and cell surface processes. We have developed a facile method for synthesis of cholesterylated peptides, each bearing a carbohydrate moiety and a fluorescent tag. The position of the cholesterol moiety on the peptide can be controlled by using a new Fmoc-protected cholesterol-triazole-lysine group, which we constructed by means of solid-phase peptide synthesis. We succeeded in integrating the glyco modules into giant unilamellar vesicles by electroformation or infusion in buffer solution. The glyco-decorated liposomes were recognized by a lectin and had unique topological membrane features. In conclusion, this work is a proof of principle for the functionalization of artificial membranes with a primitive synthetic glycocalyx useful for studying carbohydrate-protein interactions on a simplified cell-like membrane surface.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据