期刊
JOURNAL OF ANALYTICAL CHEMISTRY
卷 75, 期 2, 页码 254-261出版社
PLEIADES PUBLISHING INC
DOI: 10.1134/S106193482002015X
关键词
electrografting; label-free immunosensor; measles antigen; covalent immobilization
资金
- Government of the Russian Federation [211]
- [A03.21.0006]
The effect of the method of immobilization of receptor layer on the analytical characteristics of label-free electrochemical immunosensors for determining antibodies to the measles virus is estimated. The receptor layer bearing the measles antigen was immobilized by dropping a model suspension on the surface of a glassy-carbon electrode, followed by drying in air and covalent crosslinking by electrografting of p-nitrophenyldiazonium chloride using glutaraldehyde as a crosslinking agent. The dependences of the peak current of the mediator system on the concentration of measles antibodies in model suspensions are linear and described by the equations I = -(28 +/- 1)c(at) - (30 +/- 4) and I = -(19 +/- 2)c(at) - (15 +/- 5) for the covalent immobilization and drop deposition of the receptor layer, respectively. Immobilization of the bioreceptor by covalent crosslinking does not lead to a significant improvement in the sensitivity of the determination; however, it expands the linear concentration range tenfold and decreases the total duration of the immunoassay procedure to 20 min with respect to the immunosensor with a drop-immobilized antigen.
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