Vagococcus xieshaowenii sp. nov., isolated from snow finch (Montifringilla taczanowskii) cloacal content

A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49(T), was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China. Analysis of the 16S rRNA gene sequence showed that strain CF-49(T) was closely related to Vagococcus elongatus CCUG 51432(T) (96.5% similarity), Vagococcus fluvialis NCFB 2497(T) (96.0%) and Vagococcus lutrae CCUG 39187(T) (95.9%). whereas the similarity to another isolate (CF-210) was 99.9%. Strains CF-49(T) and CF-210 grew optimally at 37 degrees C and pH 7.0 and in the presence of 0.5% (w/v) NaCl. Acid was produced from N-acetylglucosamine, cellobiose, D-fructose. D-glucose, D-mannose, D-mannitol, maltose, D-ribose and salicin. The cell-wall peptidoglycan type was A4 alpha (L-Lys-D-Asp). The major cellular fatty acids (>10%) were C-16(:0) (35.6%), C-14:0 (17.3%). C-18:1 omega 9c (16.2%) and C-16:1 omega 9c (10.6%). The predominant respiratory quinone was menaquinone MK-7 (68.8%). The G+C content of the genomic DNA was 35.9 mol%. Digital DNA-DNA hybridization of strain CF-49(T) with V. fluvialis DSM 5731(T). V. elongatus CCUG 51432(T) and V lutrae CCUG 39187(T) resulted in relatedness values of 21.4, 23.3 and 24.6%, respectively. Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus Vagococcus. with the name Vagococcus xieshaowenii. The type strain is CF-49(T) (=CGMCC 1.6436(T) =GDMCC 1.1588(T)=JCM 33477(T)).