期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 56, 期 5, 页码 1262-1273出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2020.5009
关键词
CAP; PAS; BCC; ROS; RNA-sequencing; MAPK
类别
资金
- Foundation of The Second Affiliated Hospital of Anhui Medical University [2015hhjh04]
- National Natural Science Foundation of China [51777206, 31600680, 81673099, 31670860, 81602083, 31800702]
- Anhui Provincial Natural Science Foundation [1608085QH216, 1708085MA13]
- Grants for Scientific Research of BSKY from Anhui Medical University [XJ201505]
Basal cell carcinoma is a common skin tumor. Cold atmospheric plasma (CAP) has attracted increasing attention for its antitumor effects. The aim of the present study was to investigate the effects and related mechanisms of two CAP-activated solutions on the TE354T basal cell carcinoma and HaCat keratinocyte cell lines. Plasma-activated solution (PAS) was prepared by CAP irradiation of DMEM and PBS. TE354T cells were treated with PAS in vitro and the effect on cell viability was evaluated by an MTT assay. The apoptosis rate was detected by Annexin V/propidium iodide staining. Furthermore, western blotting and RNA-sequencing were performed. The present results demonstrated that PAS induced apoptotic signaling in basal cell carcinoma cells, and that this effect was associated with the activation of the MAPK signaling pathway. Therefore, the present study demonstrated that PAS may serve as a novel treatment for basal cell carcinoma.
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