期刊
INTERNATIONAL JOURNAL OF NEUROSCIENCE
卷 131, 期 9, 页码 864-874出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00207454.2020.1759588
关键词
NF-kappa B; Alzheimer's disease; amyloid beta; PI3K; AKT; mTOR signaling pathway
This study found increased concentration of molecules related to inflammatory response in AD patients and observed that ROF treatment could decrease expression of apoptosis-related molecules by inhibiting the PI3K/AKT/mTOR signaling pathway, as well as increase key enzyme expression in the tricarboxylic acid (TCA) cycle in SH-SY5Y cells. Inhibition of NF-kappa B could enhance these effects, while overexpression of NF-kappa B could diminish them.
Alzheimer disease (AD) is a progressive neurodegenerative disease and mostly endanger the health of people older than 65 years. Accumulation of beta amyloid protein (A beta) is the main characteristic of AD. Roflupram (ROF) could improve the behavior of AD in a mouse model. In this study, we first detected the increased concentration of molecules related to inflammatory response in serum sample of patients with AD. Next, a cell model of nuclear factor kappa B (NF-kappa B) inhibition and NF-kappa B overexpression was established in SH-SY5Y cells, A beta was used to simulate the toxicity to cells. ROF treatment decreased expression of apoptosis-related molecules via inhibition of PI3K/AKT/mTOR signaling pathway, decreased expression of pro-inflammatory factors, and increased expression of key enzymes in the tricarboxylic acid (TCA) cycle was observed in SH-SY5Y cells after ROF treatment. Inhibition of NF-kappa B could enlarge these trends whereas overexpression of NF-kappa B could reduce these trends.
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