4.7 Article

TATA-Like Boxes in RNA Polymerase III Promoters: Requirements for Nucleotide Sequences

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出版社

MDPI
DOI: 10.3390/ijms21103706

关键词

RNA polymerase III; promotor; TATA box; transcription factor; ncRNA gene; SINE

资金

  1. Russian Foundation for Basic Research [17-04-00686-a, 19-14-00327]
  2. Russian Scientific Foundation

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tRNA and some other non-coding RNA genes are transcribed by RNA polymerase III (pol III), due to the presence of intragenic promoter, consisting of boxes A and B spaced by 30-40 bp. Such pol III promoters, called type 2, are also intrinsic to Short Interspersed Elements (SINEs). The contribution of 5 ' -flanking sequences to the transcription efficiency of genes containing type 2 promoters is still studied insufficiently. Here, we studied this issue, focusing on the genes of two small non-coding RNAs (4.5SH and 4.5SI), as well as B1 and B2 SINEs from the mouse genome. We found that the regions from position -31 to -24 may significantly influence the transcription of genes and SINEs. We studied the influence of nucleotide substitutions in these sites, representing TATA-like boxes, on transcription of 4.5SH and 4.5SI RNA genes. As a rule, the substitutions of A and T to G or C reduced the transcription level, although the replacement of C with A also lowered it. In 4.5SH gene, five distal nucleotides of -31/-24 box (TTCAAGTA) appeared to be the most important, while in the box -31/-24 of 4.5SI gene (CTACATGA), all nucleotides, except for the first one, contributed significantly to the transcription efficiency. Random sequences occurring at positions -31/-24 upstream of SINE copies integrated into genome, promoted their transcription with different efficacy. In the 5 ' -flanking sequences of 4.5SH and 4.5SI RNA genes, the recognition sites of CREB, C/EBP, and Sp1 factors were found, and their deletion decreased the transcription.

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