期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 46, 期 2, 页码 633-640出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2020.4607
关键词
cardiac fibrosis; human ventricular fibroblasts; transforming growth factor-beta 1; atorvastatin; extracellular matrix; signaling pathways
资金
- Natural Science Foundation of Southwest Medical University
- Foundation of The Affiliated Hospital of Southwest Medical University [2017-PT-43]
Excessive proliferation and myofibroblasts transformation of cardiac fibroblasts play a critical role in the process of cardiac fibrosis. Atorvastatin (ATV), a 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase inhibitor, is commonly used to treat hypercholesterolemia. It has previously been shown that ATV has potential anti-fibrotic effects. However, the underlying mechanisms of ATV against cardiac fibrosis remain to be fully elucidated, and to the best of our knowledge, there are no reports focusing on the effects of ATV on transforming growth factor-beta 1 (TGF-beta 1)-induced human ventricular fibroblasts (hVFs) activation. In the present study, hVFs were stimulated with TGF-beta 1 with or without pretreatment with ATV. Subsequently, hVF proliferation, cytotoxicity, myofibroblast differentiation and pro-fibrotic gene expression were assessed. Canonical and non-canonical signaling downstream of TGF-beta 1, such as Smad3 and mitogen-activated protein kinase (MAPK) signaling, were investigated by evaluating the phosphorylation levels of Smad3, extracellular signal-regulated kinase 1/2, p38 MAPK and c-Jun N-terminal kinase. The results indicated that ATV significantly prevented TGF-beta 1-induced cell proliferation, myofibroblast differentiation and production of extracellular matrix proteins, such as matrix metalloproteinase-2, collagen I and collagen III, in hVFs. Furthermore, ATV effectively inhibited TGF-beta 1-induced activation of Smad3 and MAPK signaling in hVFs. In conclusion, the present results demonstrated that ATV prevented TGF-beta 1-induced fibrogenesis in hVFs, at least in part by inhibiting the Smad3 and MAPK signaling pathways. Therefore, these results imply that ATV may be a promising agent to treat myocardial fibrosis.
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