期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 151, 期 -, 页码 545-553出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.02.068
关键词
Degradation; Fucoidan; Epithelial-mesenchymal transition; Kidney fibrosis
资金
- National Key Research and Development Program of China [2018YFD0901104]
- Major Scientific & Engineering Projects of Innovation in Shandong Province [2019JZZY010818]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2016190]
- K. C. Wong Education Foundation, CAS
In order to investigate the anti-fibrotic effect of different molecular weight (Mw) fucoidans on TGF-beta 1-induced mouse renal tubular epithelial cell (MTEC) mode. Oxidative degradation method was used to obtain fucoidans with different molecular weights and the reaction time, reaction temperature and the concentration of oxidants were investigated. Cell viability was detected by CCK-8, and EMT markers expression was detected by Westernbolt and Cell immunofluorescence assay. As a result, after chemical analysis of three independent batches of prepared samples, one batch of fucoidan sample (LHX 1-9) which chemical contents are similar but Mw ranging from 3.3 KDa to 49.3 KDa were selected to do further research. We found LHX1 (Mw - 3.3 KDa) and LHX 3-9 (Mw = 6.6 KDa, 8.3 KDa, 11.3 KDa, 14.9 KDa, 25.2 KDa, 35.4 KDa, 49.3 KDa) could resist the TGF-beta 1-induced depithelial-mesenchymal transition (EMT) by decreased expression of Fn and CTGF and maintained epithelial cell morphology in MTEC. However, the relationship between the Mw of fucoidans and their anti-EMT effect is not simply linear. Among the samples, LHX 1, 5 and 8 showed significant anti-EMT effects than others by deregulated Fn and CTGF expression on MTEC cells. (C) 2020 Elsevier B.V. All rights reserved.
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