期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 160, 期 -, 页码 889-902出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.05.174
关键词
Lipases; Yarrowia lipolytica; Magnetic nanoparticles; Immobilization
资金
- CAPES (Brazil)
- CNPq (Brazil)
- FAPERJ (Brazil)
- CNPq
- Faperj
Magnetic nanoparticles (Fe3O4) were used for physical adsorption of lipase from Yarrowia lipolytica IMUFRJ 50682. The optimal adsorption conditions were obtained as follows: enzyme/support 19.3 mg/g and temperature of 20 degrees C for standard protein. High immobilization efficiency of 99% was obtained for 4 mL of crude lipase extract (containing 0.315 mg protein/mL) and 0.02 g of magnetic nanoparticles and this biocatalyst was recycled 30 times with 70% of lipase activity in the end. Purified lipase extracts were also efficiently immobilized and ultrafiltered lipase extract (ULE) and aqueous two-phase system lipase extract (ATPS_LE) when immobilized revealed higher hydrolytic activity in relation to CLE (2.8 and 4.0 times higher, respectively). Broad pH tolerance and high thermostability could be achieved by immobilization on magnetic nanoparticles, with 40% improvement in thermodynamic parameters at 60 degrees C. Kinetic parameters V-max and K-m were also better for ULE (V-max: 2.3 times higher; K-m 43% reduction) and ATPS_LE (V-max: 3.0 times higher; K-m: 38% reduction) immobilized on magnetic nanoparticles in relation to CLE. These results showed that the immobilization of lipase onto magnetic nanoparticles by physical adsorption is an efficient and simple way to obtain a great catalyst. (C) 2020 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据