期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 151, 期 -, 页码 663-676出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.02.160
关键词
Glycosaminogjycans; Silver-banded whiting; Chondroitin sulfates; Hyaluronic acid; Biocompatibility
资金
- USAID STRIDE - CARWIN (United States Agency for International Development Science, Technology, Research and Innovation for Development -Collaborative Applied Researchwith Industry) program [0213997-G-2015-012-00, 0213997-G2015-012-05]
- Matsumae International Fellowship Program (2019-2020)
- University of Mississippi
- American Association of Colleges of Pharmacy 2019 New Investigator Award
Glycosaminoglycans (GAGs) were extracted from heads of silver-banded whiting (SBW) fish and subjected to preliminary biocompatibility testing per ISO 10993: intracutaneous irritation, maximization sensitization, systemic toxicity, and cytotoxicity. When the GAG solution was injected intradermally. the observed irritation was within ISO limits and comparable to a marketed control. There was no evidence of sensitization, systemic toxicity, or cellular toxicity on the test organisms treated with the GAG mixture from SOW fish heads. Fractionation by size-exclusion chromatography has shown three distinct fractions: F1 as low molecular weight hyaluronic acid (190 kDa), 12 (82 kDa) and F3 (64 kDa ), both as chondroitin sulfates. Structural characterization by 1D and 2D nuclear magnetic resonance spectroscopy and disaccharide analysis have shown sulfation ratios at positions C4:C6 of the 12 and F3 fractions respectively as 70:20% and 50:30%, and the balance of non-sulfated and 4,6-di-sulfated units. The preliminary results here suggest that GAG-based extracts from SBW fish heads are suitable alternative products to be used in soft tissue augmentation, although further long-term biocompatibility studies are still required. (C) 2020 Elsevier B.V. All rights reserved.
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