4.6 Article

Inhibition of trehalase affects the trehalose and chitin metabolism pathways in Diaphorina citri (Hemiptera: Psyllidae)

期刊

INSECT SCIENCE
卷 28, 期 3, 页码 718-734

出版社

WILEY
DOI: 10.1111/1744-7917.12819

关键词

chitin metabolism; Diaphorina citri; RNA interference; trehalase; validamycin

资金

  1. National Natural Science Foundation of China [31960116]
  2. Educational Commission of Jiangxi Province of China [GJJ180747]
  3. National Key Research and Development Program of China [2018YFD0201504]
  4. earmarked fund for Jiangxi Agriculture Research System [JXARS-07]
  5. Key Research and Development Plan of Jiangxi Province [20192BBF60057]

向作者/读者索取更多资源

The study identified three trehalase genes in the Asian citrus psyllid, revealing their important role in regulating chitin metabolism and providing a new target for control of the psyllid.
The Asian citrus psyllid, Diaphorina citriis the principal vector of huanglongbing, which transmits Candidatus Liberibacter asiaticus. Trehalase is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and development. The specific functions of this enzyme in D. citrihave not been determined. In this study, three trehalase genes (DcTre1-1, DcTre1-2, and DcTre2) were identified based on the D. citri genome database. Bioinformatic analysis showed that DcTre1-1 and DcTre1-2 are related to soluble trehalase, whereas DcTre2 is associated with membrane-bound trehalase. Spatiotemporal expression analysis indicated that DcTre1-1 and DcTre1-2 had the highest expression levels in the head and wing, respectively, and DcTre2 had high expression levels in the fat body. Furthermore, DcTre1-1 and DcTre1-2 expression levels were induced by 20-hydroxyecdysone and juvenile hormone III, but DcTre2 was unaffected. The expression levels of DcTre1-1, DcTre1-2, and DcTre2 were significantly upregulated, which resulted in high mortality after treatment with validamycin. Trehalase activities and glucose contents were downregulated, but the trehalose content increased after treatment with validamycin. In addition, the expression levels of chitin metabolism-related genes significantly decreased at 24 and 48 h after treatment with validamycin. Furthermore, silencing of DcTre1-1, DcTre1-2, and DcTre2 reduced the expression levels of chitin metabolism-related genes and led to a malformed phenotype of D. citri. These results indicate that D. citritrehalase plays an essential role in regulating chitin metabolism and provides a new target for control of D. citri.

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