Extraction and antioxidant activities in vivo of pumpkin polysaccharide

Pumpkin polysaccharide was extracted with hot water. Then the protein in the polysaccharide was removed by Sevage method, and finally the pumpkin polysaccharide (P) was obtained by dialysis and column chromatography separation. The content of polysaccharide reached 96.8 %. The sugar chain in pumpkin polysaccharide had a-glycoside bond. The pumpkin polysaccharide could significantly increase the protein content, GSH-Px activity and SOD activity in serum, liver, spleen and kidney of mice, and also significantly reduce the MDA content in serum, liver, spleen and kidney of mice. Therefore, pumpkin polysaccharide has better antioxidant activity in vivo. This provides a basis for the development and utilization of antioxidant food.