期刊
IMMUNITY
卷 52, 期 3, 页码 557-+出版社
CELL PRESS
DOI: 10.1016/j.immuni.2020.02.001
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资金
- Stanford Human Immune Monitoring Center (NIH S10 Shared Instrumentation) [S10RR027431-01]
- Swedish Research Council
- Knut and Alice Wallenberg Foundation
- Novo Nordisk Foundation [NNF14CC0001]
- Lundbeck Foundation [R155-2014-4184]
- Swedish Research Council [2017-02072]
- Swedish Cancerfonden [18 0598]
- NIH [R01 DK101119]
- Swedish Research Council [2017-02072] Funding Source: Swedish Research Council
The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1(+) high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.
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