期刊
BIOTECHNOLOGY LETTERS
卷 37, 期 11, 页码 2247-2253出版社
SPRINGER
DOI: 10.1007/s10529-015-1903-4
关键词
5-Aminolevulinic acid; 5-Aminolevulinic acid synthase; Enzyme purification; Thermostability
资金
- National Key Basic Research Program of China (973 Program) [2012CB725203, 2011CBA00804]
- National High Technology Research and Development Program of China (863 Program) [2012AA022103]
- Natural Science Foundation of Tianjin [12JCYBJC33000]
As 5-aminolevulinic acid synthase (ALAS), the key enzyme for 5-aminolevulinic acid (ALA) synthesis, is unstable, we have sought to find thermostable ALASs from thermophilic organisms. Three ALASs from thermophiles Geobacillus thermoglucosidasius (GT-ALAS), Laceyella sacchari (LS-ALAS) and Pseudomonas alcaliphila (PA-ALAS) were purified and characterized. All enzymes were more stable than two previously studied ALASs from Rhodopseudomonas palustris and Rhodobacter sphaeroides. There was almost no activity change after 60 h at 37 A degrees C for the three thermostable enzymes. This contrasts with the other two enzymes which lost over 90 % activities in just 1 h. Furthermore, the specific activity of LS-ALAS (7.8 U mg(-1)) was also higher than any previously studied ALASs. Thermostable ALASs were found in thermophilic organisms and this paves the way for developing cell free processes for enzymatic production of ALA from bulk chemicals succinate and glycine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据