期刊
HYDROBIOLOGIA
卷 847, 期 15, 页码 3201-3211出版社
SPRINGER
DOI: 10.1007/s10750-020-04304-z
关键词
Groundwater; Single-species detection assays; Metabarcoding; Environmental Impact Assessment; Monitoring; Stygofauna
资金
- Australian Research Council (ARC) (South Australian Museum) [LP140100555]
- Australian Research Council (ARC) (Western Australian Museum) [LP140100555]
- Australian Research Council (ARC) (Department of Biodiversity, Conservation and Attractions) [LP140100555]
- Australian Research Council (ARC) (Bennelongia Pty Ltd) [LP140100555]
- Australian Research Council (ARC) (Biota Environmental Sciences Pty Ltd) [LP140100555]
- ARC (Rio Tinto) [LP190100555]
- ARC (BHP) [LP190100555]
- ARC (Chevron) [LP190100555]
- ARC (South Australian Museum) [LP190100555]
- ARC (Western Australian Museum) [LP190100555]
- ARC (Department of Biodiversity, Conservation and Attractions) [LP190100555]
- ARC (Department for Water and Environmental Regulation) [LP190100555]
- ARC (Western Australian Biodiversity Science Institute (WABSI)) [LP190100555]
- Rio Tinto consulting contract
- WABSI - Rio Tinto
- BHP
- Australian Research Council [LP190100555] Funding Source: Australian Research Council
The blind cave eel,Ophisternon candidum(Mees in J R Soc West Aust 45: 24-32,1962), is a rare groundwater inhabitant found in geographically isolated populations of north-west Australia. The species is listed as vulnerable under Commonwealth legislation and is a priority consideration when environmental disturbance by resource companies is proposed. Detection of this species for Environmental Impact Assessment (EIA) and monitoring is difficult because individuals are naturally rare or traditional sampling techniques are ineffective. To properly manage the species, information on population distribution and connectivity is critical. We sought to examine whether environmental DNA (eDNA) ofO. candidumcould be detected and whether positive detection was correlated with previous locations where the species had been physically caught. We developed new eDNA species-specific PCR assays to screen groundwater sampling points and we detectedO. candidumDNA in three boreholes where the species has previously been collected and five additional groundwater sampling points. Our results demonstrated that the newly designed assays were effective for detecting this rare and vulnerable subterranean species. This work sets a benchmark for the application of eDNA species-specific PCR assays for EIA and monitoring, and has potential for these assays to be expanded more broadly to high-throughput eDNA metabarcoding for subterranean groundwater communities in the future.
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