期刊
GENESIS
卷 58, 期 5, 页码 -出版社
WILEY
DOI: 10.1002/dvg.23359
关键词
fibroblast; Fsp1; Flpo; mice; recombinase; transgenic
资金
- Agence Nationale de la Recherche
- Ligue Nationale Contre le Cancer
- Ministere de l'Enseignement Superieur et de la Recherche of France
- Ligue Nationale Contre le Cancer, comite departemental de l'Ain and comite de la Saone et Loire
- Association pour la Recherche sur le Cancer [ARC2010]
- Centre National de la Recherche Scientifique
- Institut National de la Sante et de la Recherche Medicale
- Institut National du Cancer -Institut National du Cancer -PAIR PANCREAS [PAN17-045]
Recombination systems represent a major breakthrough in the field of genetic model engineering. The Flp recombinases (Flp, Flpe, and Flpo) bind and cleave DNA Frt sites. We created a transgenic mouse strain ([Fsp1-Flpo]) expressing the Flpo recombinase in fibroblasts. This strain was obtained by random insertion inside mouse zygotes after pronuclear injection. Flpo expression was placed under the control of the promoter of Fsp1 (fibroblast-specific protein 1) gene, whose expression starts after gastrulation at Day 8.5 in cells of mesenchymal origin. We verified the correct expression and function of the Flpo enzyme by several ex vivo and in vivo approaches. The [Fsp1-Flpo] strain represents a genuine tool to further target the recombination of transgenes with Frt sites specifically in cells of mesenchymal origin or with a fibroblastic phenotype.
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