期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 289, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2020.113394
关键词
Chiral chromatography; Diapause; Heteroptera; Juvenile hormone III skipped bisepoxide (JHSB(3)); Ultraperformance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS)
资金
- JSPS KAKENHI [24580087]
- Grants-in-Aid for Scientific Research [24580087] Funding Source: KAKEN
Juvenile hormone (JH) plays a pivotal role in many aspects of insect physiology. Although its presence was first reported in a blood-sucking bug belonging to the suborder Heteroptera (true bugs), JH species in the group has long been controversial. Although some recent studies proposed a putative JH molecular species in several Heteropteran species, it is not conclusive because physicochemical analyses were insufficient in most cases. Here, we studied this issue with an ultraperformance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) equipped with C18 and chiral columns in the bean bug Riptortus pedestris (Heteroptera, Alydidae), in which the JH species has long been controversial. Although a recent study describes JHSB(3) as the major JH of this species, that finding was not conclusive because its chirality has not been clarified. In the present study, we detected methyl (2R,3S,10R)-2,3;10,11-bisepoxyfamesoate, commonly named juvenile hormone III skipped bisepoxide (JHSB(3)), in the culture media of the corpora cardiaca-corpus allatum (CC-CA) complex and in the hemolymph of this species by a chiral ultraperformance liquid chromatography- tandem mass spectrometer (UPLC-MS/MS). Other JHSB(3) stereoisomers were not detected. Topical application of JHSB(3) effectively averted diapause. These results indicate that JHSB(3) is the major JH of R. pedestris. The present study further revealed that JHSB(3) and its (2R,3S,10S) isomer are more potent than (2S,3R,10R) and (2S,3R,10S) isomers, which suggests that there is a significance to the configuration of the 2,3-epoxide moiety in JH action. We further found a supplemental significance to the configuration of the 10-position.
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