期刊
BIOTECHNOLOGY LETTERS
卷 37, 期 10, 页码 2083-2090出版社
SPRINGER
DOI: 10.1007/s10529-015-1880-7
关键词
Mealy bug; Phenacoccus solenopsis; Potato virus X; Recombinant virus; RNA interference; Solenopsis mealybug
资金
- Higher Education Commission of Pakistan
The chitin synthase 1 (CHS1) gene in Phenacoccus solenopsis (PsCHS1) was evaluated as a potential target of RNA interference (RNAi) by using Potato virus X (PVX) as a vector (recombinant PVX) for expressing RNAi triggering elements in Nicotiana tabacum L. RT-PCR analysis confirmed the expression of PsCHS1 in N. tabacum inoculated with recombinant-PVX-PsCHS1 (treated). RT- and multiplex-PCR further showed a reduction in mRNA levels of the target gene in mealybugs feeding on treated plants. Mortality in parent adults and emerging nymphs (21 and 29 %) exposed to the treated plants was significantly higher (P < 0.05) than those exposed to uninoculated (-ve control) or inoculated with non-recombinant PVX (PVX-control). The number of surviving adults and the combined number of adults and nymphs (47 and 60 %) was significantly (P < 0.05) lower on the treated plants than the -ve (76 %) or PVX (74 %) control. The visual observations verified the physical deformities in mealybugs exposed to the treated plants. chitin synthase 1 is a potential RNAi target in P. solenopsis and the recombinant PVX can be used as a tool to evaluate candidate RNAi triggering elements in plants.
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