期刊
FOOD CHEMISTRY
卷 310, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125955
关键词
Vibrio parahaemolyticus; Strand replacement primer; Pyrophosphatase; Phosphate; Visualization
资金
- National GMO Cultivation Major Project of New Varieties [2018ZX08011-02B]
- National Natural Science Foundation Project of China [31871875]
Vibrio parahaemolyticus is a major hidden danger of food safety. To develop a rapid, sensitive and on-site detecting method of Vibrio parahaemolyticus (V. parahaemolyticus), a strand replacement primer thermostat phosphate (SRPP) visual sensor was proposed, based on Bst DNA polymerase and pyrophosphatase. The novel strand replacement primer (SRP) facilitates chain substitution and to open a self-folding hairpin by adding region at its 3' end. Under the action of the SRP, a pair of external primers and two inner primers, target DNA is specifically amplified at 63 degrees C relies mainly on the hairpin. Many pyrophosphates (PPi) are simultaneously generated as by-products, which can be converted into phosphates (Pi) by pyrophosphatase for phosphomolybdate blue visual detection within 5 min. The proposed biosensor can detect 1.29 x 10(3) copies of V. parahaemolyticus within 35 min.
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