期刊
FOOD CHEMISTRY
卷 310, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125942
关键词
Foodborne pathogen; Salmonella enteritidis; Nanobody; Sandwich ELISA; Immunoassay
资金
- Fund of Guangdong Provincial Key Laboratory of Food Quality and Safety, China [2018KF001]
- National Natural Science Foundation of China [31972157, 31501560]
- National Key R&D Program of China [2016YFE0106000]
- Shaanxi Provincial Research Fund [2016SF-242]
- Fundamental Research Funds for the Central Universities [2452015207]
- China Postdoctoral Science Foundation [2019M653767]
The variable domain of heavy chain only antibody (VHH), also named as nanobody obtained from camelid antibody libraries, has shown great potential in immunochemistry, with many advantages over conventional antibodies. Here, nanobodies towards Salmonella enteritidis (S. enteritidis) were isolated for the first time after biopanning of an immune camelid nanobody library. Those nanobodies showed high thermostability and good specificity. A sandwich-ELISA was developed with nanobody Nb 13, which exhibited a detection limit of 1.4 x 10(5) CFU/mL. The proposed assay was also applied to detect S. enteritidis in milk samples, which could detect 6 CFU/mL of S. enteritidis in milk after 10 h of enrichment. This study demonstrated an alternative strategy for S. enteritidis determination, and further proved the utility and practicality of nanobody-based reagents in bioanalytical chemistry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据