期刊
FOOD CHEMISTRY
卷 313, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.126130
关键词
Structured lipids; Lipozyme RM IM; Orthogonal design; Interesterification reaction; Acyl migration; Molecular dynamics simulation
资金
- National Key Research and Development Program of China [2016YFD0401404]
- National Natural Science Foundation of China [31571870, 31460427]
- Research Program of State Key Laboratory of Food Science and Technology [SKLF-ZZB-201709]
New structured lipids with 1,3-oleic-2-medium chain (OMO) triacylglycerols were synthesized by promoting acyl migration in Lipozyme RM IM catalyzed interesterification between coconut oil (CO) and high oleic rapeseed oil (HORO). Results from an orthogonal design L-25(5(5)) showed that the maximal yield of OMO-structured triacylglycerols was 45.65% under the following conditions: the molar ratio of CO to HORO, 50:50; enzyme dosage, 12 wt%; reaction temperature, 60 degrees C; reaction time, 2 h; water activity, 0.07. Low water activity showed a high rate of acyl migration (10.86% vs 5.07% no water system), which promoted OMO synthesis due to medium-chain fatty acid migration to the sn-2 position. In a low water content (5%) system of the molecular dynamics simulation, water molecules stabilized the whole structure of RM IM through hydrogen bonding, which helped fix lipase-catalyzed active sites, making substrates more easily inserted into active sites, resulting in increased enzyme activity.
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