期刊
FOOD CHEMISTRY
卷 311, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.126007
关键词
Spatial-resolution; Targeted metabolomics; LC-MS/MS; Tea; Mechanical piercing; Wounding
资金
- National Natural Science Foundation of China, China [31972467]
- Central Public-Interest Scientific Institution Basal Research Fund, China [1610212018009, Y2019PT20-02]
- Earmarked Fund for China Agricultural Research System, China [CARS-19]
In this study, a novel spatial-resolution targeted metabolomics method was developed for a single leaf on the basis of microscale sample preparation and dansylation derivatization coupled with ultraperformance liquid chromatography-tandem mass spectrometry with a spatial resolution of 1.7 x 1.7 mm(2) . The practicability of this method was demonstrated by providing absolute quantitative within-leaf distribution information for 56 endogenous metabolites (including 8 catechins, 2 alkaloids, theanine, 4 theaflavins, 14 flavonol/flavone and their glycosides, 21 amino acids, and 6 phenolic acids). An application of this method in a mechanically pierced tea leaf indicated that astringent catechins, quercetin, and quercetin glycosides may be involved in the tea plant response to the wounding. In conclusion, the proposed novel method offered richest information on the withinleaf distribution of metabolites in tea to date and will be greatly helpful in understanding the defensive responses of tea plants against biotic and abiotic stresses.
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