期刊
BIOTECHNOLOGY LETTERS
卷 37, 期 11, 页码 2295-2302出版社
SPRINGER
DOI: 10.1007/s10529-015-1917-y
关键词
Caffeic acid 3-O-methyltransferase; Ferulic acid; Heterologous expression; Ligusticum chuanxiong; Molecular cloning
资金
- National Natural Science Foundation of China [31371232]
- National Science and Technology Major Project [2014ZX09304307001-019]
- Fundamental Research Funds for the Central Universities of China [2682014RC14]
To clone and characterize caffeic acid 3-O-methyltransferase (LcCOMT) from the rhizome of Ligusticum chuanxiong, a traditional medicinal herb having a high content of ferulic acid. LcCOMT encoded an ORF of 362 amino acids with a calculated MW of 39,935 Da and pI of 5.94. Polygenetic tree indicated that LcCOMT was attributed to a new member of COMTs in plants. The recombinant LcCOMT was expressed in E. coli. HPLC and H-1 NMR analyses of purified LcCOMT protein confirmed that it could catalyze caffeic acid to produce ferulic acid in vitro. The further site-mutagenesis proved that His268 was one key catalytic residue. In addition, the substantial changing expression level of LcCOMT under chilling treatment suggested that LcCOMT might play important role in the accumulation of ferulic acid under chilling treatment. This is the first report of the isolation and characterization of a COMT clone from traditional medicine containing high contents of pharmaceutical ferulic acid.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据