期刊
CELLULOSE
卷 24, 期 1, 页码 35-47出版社
SPRINGER
DOI: 10.1007/s10570-016-1098-4
关键词
Mannan; Cellulose; Adsorption; Mannanase; Enzymatic hydrolysis
资金
- Natural Science Foundation of China [31270622]
During the pretreatment of lignocellulosic materials, the dissolved mannan would re-adsorb on cellulose, and then inhibited the cellulose hydrolysis by cellulases. However, the adsorption of mannan on cellulose and hydrolyzability of mannan adsorbed on cellulose were not so clear. In this work, the adsorption behavior of mannans on cellulose and the hydrolysis of adsorbed mannan by mannanase were investigated. Adsorption of 1, 4-beta-D-mannan (mannan), Konjac glucomannan (GM), and Carob galactomannan (GalM) on Avicel and corn stover (CS) was increased with mannan loading. The adsorbed amount of mannan (94.4 mg/g Avicel and 85.1 mg/g CS) on cellulosic substrates at the mannan concentration of 5 mg/mL was significantly higher (p < 0.05) than that of GM (65.7 mg/g Avicel and 63.7 mg/g CS) and GalM (44.3 mg/g Avicel and 48.7 mg/g CS). Furthermore, the NMR spectra and molecular weight analysis showed that mannan with less side groups and low molecular weight might increase the adsorption. Mannan, GM, and GalM adsorbed on Avicel and CS, which was used as Avicel/CS -mannan/GM/GalM complex, could be hydrolyzed by mannanase, and the hydrolyzability of Avicel-mannan/GalM complexes was stronger than that of Avicel-GM complex. Similarly, the reducing sugars increased by 23.2 and 54.2 % for Avicel-mannan and Avicel-GalM complexes after 48 h hydrolysis by cellulase and mannanase, respectively. The results suggested that the addition of mannanase could hydrolyze the mannan adsorbed on cellulose and potentially improved hydrolysis efficiency of cellulose in lignocelluloses. Additionally, the mannanase supplementation could be extended to the removal of mannan on pulp by mannanase and finally affecting the dissolving pulps and paper quality.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据