期刊
CELLULOSE
卷 23, 期 4, 页码 2349-2361出版社
SPRINGER
DOI: 10.1007/s10570-016-0979-x
关键词
Imaging; Transmission electron microscopy; Cellulose surface structure; Cellulase; Cellobiohydrolase; Endoglucanase
资金
- Danish Council for Strategic Research, Program Commission on Sustainable Energy and Environment [11-116772]
- Augustinus Foundation [14-4639]
- Oticon Foundation [14-3925]
- DOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (BETO)
It is well documented that the enzymatic hydrolysis of cellulose follows a reaction pattern where an initial phase of relatively high activity is followed by a gradual slow-down over the entire course of the reaction. This phenomenon is not readily explained by conventional factors like substrate depletion, product inhibition or enzyme instability. It has been suggested that the underlying reason for the loss of enzyme activity is connected to the heterogeneous structure of cellulose, but so far attempts to establish quantitative measures of such a correlation remain speculative. Here, we have carried out an extensive microscopy study of Avicel particles during extended hydrolysis with Hypocrea jecorina cellobiohydrolase 1 (CBH1) and endoglucanase 1 and 3 (EG1 and EG3) alone and in mixtures. We have used differential interference contrast microscopy and transmission electron microscopy to observe and quantify structural features at mu m and nm resolution, respectively. We implemented a semi-automatic image analysis protocol, which allowed us to analyze almost 3000 individual micrographs comprising a total of more than 300,000 particles. From this analysis we estimated the temporal development of the accessible surface area throughout the reaction. We found that the number of particles and their size as well as the surface roughness contributed to surface area, and that within the investigated degree of conversion (< 30 %) this measure correlated linearly with the rate of reaction. Based on this observation we argue that cellulose structure, specifically surface area and roughness, plays a major role in the ubiquitous rate loss observed for cellulases.
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