期刊
FASEB JOURNAL
卷 34, 期 5, 页码 6418-6436出版社
WILEY
DOI: 10.1096/fj.202000196R
关键词
heavy resistance training; human; myofiber necrosis; myogenic progenitor cells; in vivo myogenesis; Sarcopenia
资金
- Nordea Foundation (Healthy Aging Grant)
- Lundbeck Foundation [R303-2018-3427]
- Danish Council for Independent Research
- Danish Agency for Culture [FPK.2016-0023]
- Bispebjerg Hospital
- Danish Rheumatism Association [R160-A5113]
- Faculty of Health and Medical Sciences at the University of Copenhagen
- AP Moller Foundation for the Advancement of Medical Science
Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20-31 years) and 19 elderly men (60-73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre- and Post-RT, and 9 days after ES, for immunohistochemistry and RT-PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P < .05), alongside necrosis and substantial changes in expression of genes related to matrix, myogenesis, and innervation (P < .05). Post-RT, VL cross-sectional area had increased in both legs (15%, P < .05) and SCs/type II fiber had increased 2-4 times more with ES+RT vs RT alone (P < .001). Together these novel findings demonstrate youthful regeneration and hypertrophy responses in human elderly muscle. Furthermore, boosting SC availability in healthy elderly men does not enhance the subsequent muscle hypertrophy response to RT.
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