期刊
CELLULOSE
卷 23, 期 2, 页码 1335-1347出版社
SPRINGER
DOI: 10.1007/s10570-016-0859-4
关键词
Psychrotrophic cellulose-degrading bacteria; Sphingobacterium sp.; CMCase activity; RSM
资金
- National Natural Science Foundation of China [41471263]
- Scientific Research Fund of Heilongjiang Provincial Education Department [12531001]
- Northeast Agricultural University Innovation Foundation for Postgraduate [yjscx14081]
Organic solid waste consists of 70 % biodegradables, such as cellulose, which is unable to be degraded quickly and efficiently in the cold region of China. Psychrotrophic cellulose-degrading bacteria play an important role in cellulose degradation at low temperatures. In this study, a psychrotrophic cellulose-degrading FLX-7 strain with high carboxymethyl cellulase (CMCase) activity was isolated from the soil of a solid waste landfill under the environmental temperature of -10 A degrees C. The FLX-7 strain was identified as Sphingobacterium sp. based on morphology and physiological and biochemical properties as well as 16S rRNA gene sequence analysis. To improve the cold-active CMCase production of FLX-7, fermentation conditions were optimized using response surface methodology based on a Box-Behnken design. The maximum CMCase activity (16.95 U/mL) was obtained at optimal fermentation conditions (inoculum size 2.1 %, temperature 10.4 A degrees C, incubation time 73.5 h, and pH 7.2). The optimum pH and temperature for enzymatic reaction of cold-active CMCase were 5.0 and 25 A degrees C, respectively. The cold-active CMCase was stable at a wide pH range of 5.0-9.0 and temperature range of 10-30 A degrees C. The FLX-7 strain could be distributed via a psychrotrophic cellulose-degrading strain library and applied more broadly to organic solid waste composting in cold regions.
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